Journal: Oncotarget

Article Title: Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis

doi: 10.18632/oncotarget.8254

Figure Lengend Snippet: ( A ) In vitro growth rate of the parental mEERL cells compared to the MLM clones. Growth is shown as doubling time (calculated as DT = Tln(2)/ln(xE/xb) where DT is doubling time; T is the time period; and xE or xB is the number of cells at the ending or beginning of the time period), each bar represents the mean ± SEM, N = 12 from three independent experiments. Differences in growth between the clones did not show statistically significant differences based on ANOVA (ns., P = 0.192). ( B ) Clonogenic survival of parental mEERL cells compared to the MLM clones. Cells were treated with 2 μM cisplatin, 4 Gy x-ray radiation or the combination of the two modalities. Experiments were repeated three times with similar results. Each bar represents an N = 8 from two independent experiments; values, means ± SEM. Statistically significant differences at day 6 after treatment, based on ANOVA: † P ≤ 0.01. ( C ) Bar graph showing cell migration and ( D ) invasion on Matrigel chambers. Bars represent an N = 7, experiments were repeated 3 times with similar results; values, means ± SEM. Statistically significant differences 12 hours after seeding, based on ANOVA: † P ≤ 0.01.

Article Snippet: For the indicated schedule, mice were anesthetized with 87.5 mg/Kg ketamine and 12.5 mg/Kg xylazine, and treated with 8 Gy X-ray radiation (RS2000 irradiator, RadSource Technologies, Inc. Suwanee, GA), intraperitoneal cisplatin (CalBiochem) dissolved in bacteriostatic 0.9% sodium chloride (Hospira Inc.) at 20 mg/m 2 or the combination of both modalities.

Techniques: In Vitro, Clone Assay, Migration

Journal: Oncotarget

Article Title: Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis

doi: 10.18632/oncotarget.8254

Figure Lengend Snippet: Parental mEERL cells and MLM clones were injected into the hind limb of C57Bl6 mice (50,000 cells/mouse N = 12 mice/group). After establishment of palpable tumors, mice were treated with IP cisplatin (20 mg/m2) and x-ray radiation (8 Gy) on days 4, 11, and 18. Individual mouse tumor growth curves for each cell line, panel ( A ) mEERL, ( B ) MLM#1, ( C ) MLM#3, ( D ) MLM#5, and ( E ) MLM#10. ( F ) Kaplan Meier tumor free survival graph. All non-surviving mice were sacrificed due to tumor burden at the primary (hind limb) tumor sight. Deaths not associated with tumor (death during CRT) were censored from the data and are indicated by dots on the corresponding curve. Statistically significant differences were calculated by pairwise multiple comparison procedures (Holm-Sidak method): † P ≤ 0.01.

Article Snippet: For the indicated schedule, mice were anesthetized with 87.5 mg/Kg ketamine and 12.5 mg/Kg xylazine, and treated with 8 Gy X-ray radiation (RS2000 irradiator, RadSource Technologies, Inc. Suwanee, GA), intraperitoneal cisplatin (CalBiochem) dissolved in bacteriostatic 0.9% sodium chloride (Hospira Inc.) at 20 mg/m 2 or the combination of both modalities.

Techniques: Clone Assay, Injection, Comparison